Phenotypic analysis of the unstimulated in vivo hiv cd4 t cell reservoir

J Neidleman; X Luo; J Frouard; G Xie; F Hsiao; T Ma; V Morcilla; A Lee; S Telwatte; R Thomas; W Tamaki; B Wheeler; R Hoh; M Somsouk; P Vohra; J Milush; KS James; NM Archin; PW Hunt; SG Deeks; SA Yukl; S Palmer; WC Greene; NR Roan

Journal article

Phenotypic analysis of the unstimulated in vivo hiv cd4 t cell reservoir

J Neidleman, X Luo, J Frouard, G Xie, F Hsiao, T Ma, V Morcilla, A Lee, S Telwatte, R Thomas, W Tamaki, B Wheeler, R Hoh, M Somsouk, P Vohra, J Milush, KS James, NM Archin, PW Hunt, SG Deeks Show all

Elife | Published : 2020

DOI: 10.7554/ELIFE.60933

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Abstract

The latent reservoir is a major barrier to HIV cure. As latently infected cells cannot be phenotyped directly, the features of the in vivo reservoir have remained elusive. Here, we describe a method that leverages high-dimensional phenotyping using CyTOF to trace latently infected cells reactivated ex vivo to their original pre-activation states. Our results suggest that, contrary to common assumptions, the reservoir is not randomly distributed among cell subsets, and is remarkably conserved between individuals. However, reservoir composition differs between tissues and blood, as do cells successfully reactivated by different latency reversing agents. By selecting 8– 10 of our 39 original Cy..

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University of Melbourne Researchers

Sushama Telwatte Author

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Funding Acknowledgements

National Institutes of Health R01AI127219 Nadia R RoanNational Institutes of Health R01AI147777 Nadia R RoanNational Institutes of Health R01AI134363 Nancie M ArchinNational Institutes of Health UM1AI126611 Steven G Deeks Sarah PalmerNational Institutes of Health P01AI131374 Warner C Greene Nadia R RoanNational Institutes of Health R01DK108349 Steven A YuklNational Institutes of Health R01AI132128 Steven A YuklNational Institutes of Health R01DK120387 Steven A YuklamfAR, The Foundation for AIDS Research 109301 Nadia R Roan Warner C Greene Steven G Deeks Steven A Yukl Peter W HuntNational Health and Medical Research Council APP1149990 Sarah Palmer